At the heart of Rtrack is the processing of the raw paths to yield descriptive metrics. Some of these metrics, such as ‘path length’ and ‘time in wall zone’ are easily explained. Others are less clear, are named differently by different authors and/or depend on varying parameters.

This document describes each of the metrics calculated by calculate_metrics and the parameters used in their definition.

The metrics object

A core functionality of the Rtrack package is the calculation of metrics from the path coordinates. The rtrack_metrics object that is returned by calculate_metrics contains the results of these calculations which are used for prediction by the machine learning model. This section explains the information contained in the rtrack_metrics object and describes the individual metrics in detail.

Object metadata

This section contains information that has been passed to the calculate_metrics function and is embedded in the rtrack_metrics object for convenience.

id

This is the track id supplied either to read_path or read_experiment and should be a unique identifier for the track. This will be used to name track analysis output and to title the plots generated by plot_path or plot_density.

arena

The rtrack_arena object associated with this track. See the function read_arena for details.

Arena zones

The active place avoidance arena is a circular space made up of several zones. The zones for this arena type are as follows:

  • The arena is defined as the circular arena in which the experiment was performed. The raw size, shape and centre point (to allow for path recording calibration) are set by the experimenter in the arena definition file/s (see the function read_arena). The fraction of time spent in this zone should be 1 (excepting only small calibration errors).
  • The centre is defined as a ring with a radius 20 % of the arena diameter, and which is centred on the centre of the arena.
  • The wall is defined as a ring with the outer extent bounded by the pool/arena and the inner radius equal to 60 % of the arena radius (i.e. the wall zone width is 20 % of the pool/arena diameter).
  • The aversive.zone is a sector of the arena that the subjects have been trained to avoid. The width of this sector is determined in the arena description file.
  • The old.aversive.zone, if present, is a sector of the arena where the aversive zone was previously located. This is used for reversal trials.
  • The quadrants. The north quadrant is the quarter of the arena area that is centred on the middle of the aversive zone. The other quadrants are defined based on this. The quadrant definitions thus change when the aversive zone is moved (such as during a reversal).

area

A list of the areas of each of the arena components. This is used in several of the metrics calculations.

path

The rtrack_path object associated with this track. See the function read_path for details.

Core metrics

This section contains all the metrics, including those calculated for all time points (such as distance from goal and heading error).

path.length

The length of the path. This is in whatever units the path was measured in (millimetres, inches, pixels).

velocity

The speed of movement at each time interval. In normalised path units per second.

immobile

A logical vector indicating whether the subject was not moving at each timepoint.

immobile.x

The x coordinates of all timepoints where the subject was immobile. This is useful for plotting the spatial locations of periods of immobility.

immobile.y

The y coordinates of all timepoints where the subject was immobile. This is useful for plotting the spatial locations of periods of immobility.

immobility

The total amount of time (in seconds) that the subject was immobile.

total.time

The total time the subject spent in the arena. This is given in seconds.

angle.from.aversive.zone

The angle, in the direction of rotation, separating the position of the subject at each timepoint from the leading edge of the aversive zone. If the subject is in the aversive zone, this angle is 0.

angle.from.old.aversive.zone

As above, but for the previous aversive zone after a reversal.

goal.crossings

The number of times the path crossed (i.e. entered and exited once) the aversive.zone.

coverage

The fraction of the arena covered by the path. This is measured by calculating the area of the minimal polygon enclosing the path (i.e. the ‘silhouette’; actually the ‘convex hull’ in technical terms) as a fraction of the area of the arena.

roaming.entropy

This is an application of the Shannon entropy measure to spatial paths. In Rtrack, it is estimated by calculating a density map with a 50 \(\times\) 50 grid (and a bandwidth of 1/50) which is clipped to the bounds of the arena. This gives a computationally fast estimate of \(p\); the likelihood of the path passing through any one of the grid cells. The entropy is then calculated using Shannon’s equation: \(-\sum(p\times log(p))\). This value is then normalised by dividing by the log of the total number of grid cells present in the arena (so that the final value ranges between 0 and 1). A roaming entropy of 1 means that the path covers the entire arena and it is impossible to predict a point on the path. A roaming entropy of 0 occurs when the entire path is within one grid cell. Lower entropy values indicate a more predictable path. The idea behind the roaming entropy measure is demonstrated at http://www.brandmaier.de/roamingentropy/.

velocity.in.zone

The speed at which the subject is moving in each zone. This is a list, with values computed for each of the defined zones.

immobility.in.zone

The total duration that the subject was immobile in each zone. This is a list, with values computed for each of the defined zones.

latency.to.zone

The time at which the subject first crossed each zone. This is a list, with values computed for each of the defined zones.

time.in.zone

The fraction of the path spent in each zone. The time at which the subject first crossed the each zone. This is a list, with values computed for each of the defined zones.

zone.crossings

The number of times each zone was traversed. This is a list, with values computed for each of the defined zones.

An overview of some of the terms used in the metrics definitions.

Summary metrics

The summary metrics are derived from the core metrics above, but are each only a single value (typically the mean).

features

This vector of (normalised) values is used for strategy calling using the machine learning model implemented in call_strategy. These are not useful for further analysis on their own.

summary

This is a selection of summary metrics that make sense for downstream analysis. These values use the same units as the raw input data. That is, the distances are in whatever units the track acquisition software used (this may be centimetres, inches or even just pixels from the video analysis—you will need to check with your acquisition software if you are unsure of what these are). Times are in the units specified in the arena description file. These will be ( must be!) the units of the timestamps in the raw acquisition data. Most of these values are medians of the metrics calculated for each point on the path1.

path.length

The total length of the path. This is just the sum of the distance between each point and the next point in the path.

total.time

The total time the subject spent in the arena. This is not necessarily the same as the maximum trial duration; e.g. in cases where the subject finds the platform early.

velocity

The median velocity. The speed of movement at each time interval. In path units per second.

immobility

The total time spent immobile.

angle.from.aversive.zone

The median angle of each point on the path from the leading edge of the aversive zone (see angle.from.aversive.zone above).

angle.from.old.aversive.zone

The median angle of each point on the path from the leading edge of the old aversive zone (see angle.from.aversive.zone above).

roaming.entropy

The roaming entropy (see roaming.entropy above).

latency.to.aversive.zone

The time at which the subject first crossed the aversive zone (see latency.to.zone above). This will be NA if the aversive zone was never entered

latency.to.old.aversive.zone

The time at which the subject first crossed the old aversive zone (see latency.to.zone above). This will be NA if the old aversive zone was never entered or if there was no old aversive zone defined.

time.in.centre.zone

Total time spent in the centre zone.

time.in.wall.zone

Total time spent in the wall zone.

time.in.aversive.zone

Total time spent in the aversive zone.

time.in.old.aversive.zone

Total time spent in the old aversive zone.

aversive.zone.crossings

The number of times the path crossed the aversive zone (see goal.crossings above).

old.aversive.zone.crossings

The number of times the path crossed the old aversive zone.

Structure of the metrics object

Below is an overview of the hierarchy of the rtrack_metrics object together with the names and classes of each component. Where the class is not part of the R base package, it is given in square brackets after the class name.

metrics : rtrack_metrics [Rtrack]
    id : character
    arena : rtrack_arena [Rtrack]
        id : character
        type : character
        description : data.frame
            type : character
            time.units : character
            arena.bounds : character
            goal : character
            (old.goal : character) **this component is optional and may be missing**
        correction : list
            t : numeric
            x : numeric
            y : numeric
            r : numeric
        pool : list
            shape : character
            x : numeric
            y : numeric
            radius : numeric
        goal : list
            shape : character
            x : numeric
            y : numeric
            radius : numeric
        old.goal : list 
            shape : character
            x : numeric
            y : numeric
            radius : numeric
        zones : list
            arena : PackedSpatVector [terra]
            centre : PackedSpatVector [terra]
            wall : PackedSpatVector [terra]
            aversive.zone : PackedSpatVector [terra]
            (old.aversive.zone : PackedSpatVector [terra]) **this component is optional and may be missing**
            n.quadrant : PackedSpatVector [terra]
            e.quadrant : PackedSpatVector [terra]
            s.quadrant : PackedSpatVector [terra]
            w.quadrant : PackedSpatVector [terra]
    area : list
        arena : numeric
        centre : numeric
        wall : numeric
        aversive.zone : numeric
        (old.aversive.zone : numeric) **this component is optional and may be missing**
        n.quadrant : numeric
        e.quadrant : numeric
        s.quadrant : numeric
        w.quadrant : numeric
    path : rtrack_path
        id : character
        raw.t : numeric
        raw.x : numeric
        raw.y : numeric
        t : numeric
        x : numeric
        y : numeric
    path.length : numeric
    velocity : numeric
    immobile : numeric
    immobile.x : numeric
    immobile.y : numeric
    immobility : numeric
    total.time : numeric
    angle.from.aversive.zone : numeric
    angle.from.old.aversive.zone : numeric
    goal.crossings : numeric
    coverage : numeric
    roaming.entropy : numeric
    velocity.in.zone : numeric
    immobility.in.zone : numeric
    latency.to.zone : numeric
    time.in.zone : numeric
    zone.crossings : numeric
    features : numeric
        path.length : numeric
        lower.velocity : numeric
        median.velocity : numeric
        upper.velocity : numeric
        lower.immobility : numeric
        median.immobility : numeric
        upper.immobility : numeric
        coverage : numeric
        turning : numeric
        absolute.turning : numeric
        lower.angle.from.aversive.zone : numeric
        median.angle.from.aversive.zone : numeric
        upper.angle.from.aversive.zone : numeric
        lower.angle.from.old.aversive.zone : numeric
        median.angle.from.old.aversive.zone : numeric
        upper.angle.from.old.aversive.zone : numeric
        roaming.entropy : numeric
        velocity.in.centre.zone : numeric
        velocity.in.wall.zone : numeric
        velocity.in.aversive.zone : numeric
        velocity.in.old.aversive.zone : numeric
        immobility.in.centre.zone : numeric
        immobility.in.wall.zone : numeric
        immobility.in.aversive.zone : numeric
        immobility.in.old.aversive.zone : numeric
        latency.to.centre.zone : numeric
        latency.to.wall.zone : numeric
        latency.to.aversive.zone : numeric
        latency.to.old.aversive.zone : numeric
        time.in.centre.zone : numeric
        time.in.wall.zone : numeric
        time.in.aversive.zone : numeric
        time.in.old.aversive.zone : numeric
        centre.zone.crossings : numeric
        wall.zone.crossings : numeric
        aversive.zone.crossings : numeric
        old.aversive.zone.crossings : numeric
    summary : numeric
        path.length : numeric
        total.time : numeric
        velocity : numeric
        immobility : numeric
        angle.from.aversive.zone : numeric
        angle.from.old.aversive.zone : numeric
        roaming.entropy : numeric
        latency.to.aversive.zone : numeric
        latency.to.old.aversive.zone : numeric
        time.in.centre.zone : numeric
        time.in.wall.zone : numeric
        time.in.aversive.zone : numeric
        time.in.old.aversive.zone : numeric
        aversive.zone.crossings : numeric
        old.aversive.zone.crossings : numeric

  1. Most of the metrics are not normally distributed so so-called parametric statistics may not be appropriate. For this reason, Rtrack defaults to using non-parametric descriptive statistics such as median and the upper/lower quartiles.↩︎